Dynamics of Transition Metal Transporting P-Type ATPases in Native Membranes

Families of soft-metal-ion-transporting ATPases.

Induction of heavy-metal-transporting CPX-type ATPases during acid adaptation in Lactobacillus bulgaricus.

Lactobacillus bulgaricus is a lactic acid bacteria (LAB) that, through the production of lactic acid, gradually acidifies its environment during growth. In the course of this process, L. bulgaricus acquires an improved tolerance to acidity. A survey of the recently established genome sequence shows that this bacterium possesses few of the pH control functions that have been described in other L...

Ion Motive ATPases: V- and P-type ATPases

Biological membranes are composed of phospholipid bilayers and mark the boundaries between the cell or organelle and its exterior. There is little, if any, free diffusion of ions or hydrophilic solutes across phospholipid bilayers. Thus, to enable the cell to control the composition of its intracellular environment, nature has evolved membrane proteins that form specialized pathways across them...

Biochemical characterization of P-type copper ATPases

Copper ATPases, in analogy with other members of the P-ATPase superfamily, contain a catalytic headpiece including an aspartate residue reacting with ATP to form a phosphoenzyme intermediate, and transmembrane helices containing cation-binding sites [TMBS (transmembrane metal-binding sites)] for catalytic activation and cation translocation. Following phosphoenzyme formation by utilization of A...

Evolution of Plant P-Type ATPases

Five organisms having completely sequenced genomes and belonging to all major branches of green plants (Viridiplantae) were analyzed with respect to their content of P-type ATPases encoding genes. These were the chlorophytes Ostreococcus tauri and Chlamydomonas reinhardtii, and the streptophytes Physcomitrella patens (a non-vascular moss), Selaginella moellendorffii (a primitive vascular plant)...